Dataset Information

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO₂.

ABSTRACT:

Background

Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO₂. Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products.

Results

In this research, an electroactive succinate-producing cell factory was engineered in E. coli T110(pMtrABC, pFccA-CymA) by expressing mtrABC, fccA and cymA from Shewanella oneidensis MR-1, which can utilize electricity to reduce fumarate. The electroactive T110 strain was further improved by incorporating a carbon concentration mechanism (CCM). This strain was fermented in an MES system with neutral red as the electron carrier and supplemented with HCO₃⁺, which produced a succinate yield of 1.10 mol/mol glucose-a 1.6-fold improvement over the parent strain T110.

Conclusions

The strain T110(pMtrABC, pFccA-CymA, pBTCA) is to our best knowledge the first electroactive microbial cell factory engineered to directly utilize electricity for the production of a specific product. Due to the versatility of the E. coli platform, this pioneering research opens the possibility of engineering various other cell factories to utilize electricity for bioproduction.

SUBMITTER: Wu Z

PROVIDER: S-EPMC6348651 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Publications

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO<sub>2</sub>.

Wu Zaiqiang Z Wu Zaiqiang Z Wang Junsong J Liu Jun J Wang Yan Y Bi Changhao C Zhang Xueli X

Microbial cell factories 20190128 1

<h4>Background</h4>Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO<sub>2</sub>. Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products.<h4>Results</h4>In this research, an electroactive succinate-producing cell factory was engineered in E. coli T110(pMtrABC, pFccA-CymA) by expressing mtrABC, fccA and cymA from Shewanella oneidensis MR- ...[more]

PMID: 30691454

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Project description:BACKGROUND: The recalcitrant nature of hemicellulosic materials and the high cost in depolymerization are the primary obstacles preventing the use of xylan as feedstock for fuel and chemical production. Consolidated bioprocessing, incorporating enzyme-generating, biomass-degrading and bioproduct-producing capabilities into a single microorganism, could potentially avoid the cost of the dedicated enzyme generation in the process of xylan utilization. In this study, we engineered Escherichia coli strains capable of exporting three hemicellulases to the broth for the succinate production directly from beechwood xylan. RESULTS: Xylanases were extracellular environment-directed by fusing with OsmY. Subsequently, twelve variant OsmY fused endoxylanase-xylosidase combinations were characterized and tested. The combination of XynC-A from Fibrobacter succinogenes S85 and XyloA from Fusarium graminearum which appeared to have optimal enzymatic properties was identified as the best choice for xylan hydrolysis (0.18 ± 0.01 g/l protein in the broth with endoxylanase activity of 12.14 ± 0.34 U/mg protein and xylosidase activity of 92 ± 3 mU/mg protein at 8 h after induction). Further improvements of hemicellulases secretion were investigated by lpp deletion, dsbA overexpression and expression level optimization. With co-expression of ?-arabinofuranosidase, the engineered E. coli could hydrolyze beechwood xylan to pentose monosaccharides. The hemicellulolytic capacity was further integrated with a succinate-producing strain to demonstrate the production of succinate directly from xylan without externally supplied hydrolases and any other organic nutrient. The resulting E. coli Z6373 was able to produce 0.37 g/g succinate from xylan anaerobically equivalent to 76% of that from xylan acid hydrolysates. CONCLUSIONS: This report represents a promising step towards the goal of hemicellulosic chemical production. This engineered E. coli expressing and secreting three hemicellulases demonstrated a considerable succinate production on the released monosaccharides from xylan. The ability to use lower-cost crude feedstock will make biological succinate production more economically attractive.

Dataset Information

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO₂.

Background

Results

Conclusions

Publications

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO<sub>2</sub>.

Similar Datasets

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Dataset Information

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2.

Background

Results

Conclusions

Publications

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO<sub>2</sub>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO₂.