Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO₂.

ABSTRACT:

Background

Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO₂. Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products.

Results

In this research, an electroactive succinate-producing cell factory was engineered in E. coli T110(pMtrABC, pFccA-CymA) by expressing mtrABC, fccA and cymA from Shewanella oneidensis MR-1, which can utilize electricity to reduce fumarate. The electroactive T110 strain was further improved by incorporating a carbon concentration mechanism (CCM). This strain was fermented in an MES system with neutral red as the electron carrier and supplemented with HCO₃⁺, whic

SUBMITTER: Wu Z 

PROVIDER: S-EPMC6348651 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature