Evaluation of FISH for Blood Cultures under Diagnostic Real-Life Conditions.

ABSTRACT:

Background

The study assessed a spectrum of previously published in-house fluorescence in-situ hybridization (FISH) probes in a combined approach regarding their diagnostic performance with incubated blood culture materials.

Methods

Within a two-year interval, positive blood culture materials were assessed with Gram and FISH staining. Previously described and new FISH probes were combined to panels for Gram-positive cocci in grape-like clusters and in chains, as well as for Gram-negative rod-shaped bacteria. Covered pathogens comprised Staphylococcus spp., such as S. aureus, Micrococcus spp., Enterococcus spp., including E. faecium, E. faecalis, and E. gallinarum, Streptococcus spp., like S. pyogenes, S. agalactiae, and S. pneumon

SUBMITTER: Reitz A 

PROVIDER: S-EPMC6348703 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature