Measurement of Average Aggregate Density by Sedimentation and Brownian Motion Analysis.

ABSTRACT: The spatially averaged density of protein aggregates is an important parameter that can be used to relate size distributions measured by orthogonal methods, to characterize protein particles, and perhaps to estimate the amount of protein in aggregate form in a sample. We obtained a series of images of protein aggregates exhibiting Brownian diffusion while settling under the influence of gravity in a sealed capillary. The aggregates were formed by stir-stressing a monoclonal antibody (NISTmAb). Image processing yielded particle tracks, which were then examined to determine settling velocity and hydrodynamic diameter down to 1 μm based on mean square displacement analysis. Measurements on polystyrene calibration microspheres ranging in size from 1 to 5 μm showed that the mean square displace

SUBMITTER: Cavicchi RE 

PROVIDER: S-EPMC6348865 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature