Extracellular esterase activity as an indicator of endoplasmic reticulum calcium depletion.

ABSTRACT:

Context

Endoplasmic reticulum (ER) calcium depletion is associated with diverse diseases, including cardiac, hepatic, and neurologic diseases.

Objective

The aim of the present study was to identify and characterize an endogenous protein that could be used to monitor ER calcium depletion comparably to a previously described exogenous reporter protein.

Materials and methods

The use of a selective esterase-fluorescein diester pair allowed for carboxylesterase activity in extracellular fluid to be measured using a fluorescent readout. Cell culture media from three different cell lines, rat plasma, and human serum all possess quantifiable amounts of esterase activity.

Results

Fluorescence produced by the interaction of carboxylesterases with a fluorescein diester su

SUBMITTER: Trychta KA 

PROVIDER: S-EPMC6348867 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature