Digestion of Human Milk Oligosaccharides by Bifidobacterium breve in the Premature Infant.

ABSTRACT:

Objective

The aim of this study was to measure consumption and absorption of human milk oligosaccharides (HMOs) in a cohort of premature infants treated with probiotic Bifidobacterium breve.

Methods

Twenty-nine premature infants (median gestational age 28 weeks, range 23-32 weeks) cared for in the neonatal intensive care unit of the King Edward and Princess Margaret Hospital in Perth, Australia, were treated with B breve at a dose of 1.66 billion organisms per day. Samples of feces, urine, and milk were obtained at initiation of the probiotic and again 3 weeks later. 16S ribosomal RNA from the feces was analyzed by next-generation sequencing. Quantitation of HMO content of the milk, urine, and feces was performed using nano-high-performance liquid chromatography-chip/time-of

SUBMITTER: Underwood MA 

PROVIDER: S-EPMC6349036 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature