Expression analysis of the polyphenol oxidase gene in response to signaling molecules, herbivory and wounding in antisense transgenic tobacco plants.

ABSTRACT:

Key message

We provide evidence that the expression of the PPO gene was significantly reduced in response to wounding, MeJ and herbivory in transgenic tobacco under wound-inducible OsRGLP2 promoter in an anti-sense orientation.

Abstract

Polyphenol oxidase (PPO) genes play an important role in plant defense mechanisms against biotic and abiotic stresses. In the present study, a 655 bp core sequence of the potato PPO gene was placed under the control of wound-inducible OsRGLP2 promoter in an anti-sense direction to evaluate its potential effects during biotic (Trialeurodes vaporariorum's infestation) and various abiotic (wounding, MeJ, ABA) stresses. Transcriptional profiling of PPO gene by real-time PCR (qRT-PCR) in transgenic tobacco

SUBMITTER: Aziz E 

PROVIDER: S-EPMC6349263 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature