The genomic landscape of UM-SCC oral cavity squamous cell carcinoma cell lines.

ABSTRACT:

Objectives

We sought to describe the genetic complexity of 14 UM-SCC oral cavity cancer cell lines that have remained uncharacterized despite being used as model systems for decades.

Materials and methods

We performed exome sequencing on 14 oral cavity UM-SCC cell lines and denote the mutational profile of each line. We used a SNP array to profile the multiple copy number variations of each cell line and use immunoblotting to compare alterations to protein expression of commonly amplified genes (EGFR, PIK3CA, etc.). RNA sequencing was performed to characterize the expression of genes with copy number alterations.

Results

The cell lines displayed a highly complex network of genetic aberrations that was consistent with alterations identified in the HNSCC TCGA project in

SUBMITTER: Ludwig ML 

PROVIDER: S-EPMC6349383 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature