The ISApl1 ₂ Dimer Circular Intermediate Participates in mcr-1 Transposition.

ABSTRACT: Objectives: The mobile colistin resistance gene mcr-1 is a serious threat to global human and animal health. The composite transposon Tn6330 and its circular intermediate were proposed to be involved in the spread of mcr-1 but their roles remain poorly understood. Methods: To further explore the intermediates during the transposition of Tn6330, we engineered Escherichia coli strains that carry an intact Tn6330 transposon or its deletion derivatives. PCR assays were performed to detect IR-IR junctions and possible circular intermediates. We carried out transposition experiments to calculate transposition frequency. The transposition sites were characterized by whole genome sequence and ISMapper-based analyses. Results: The prese

SUBMITTER: He YZ 

PROVIDER: S-EPMC6349718 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature