Accurate signal-source localization in brain slices by means of high-density microelectrode arrays.

ABSTRACT: Extracellular recordings by means of high-density microelectrode arrays (HD-MEAs) have become a powerful tool to resolve subcellular details of single neurons in active networks grown from dissociated cells. To extend the application of this technology to slice preparations, we developed models describing how extracellular signals, produced by neuronal cells in slices, are detected by microelectrode arrays. The models help to analyze and understand the electrical-potential landscape in an in vitro HD-MEA-recording scenario based on point-current sources. We employed two modeling schemes, (i) a simple analytical approach, based on the method of images (MoI), and (ii) an approach, based on finite-element methods (FEM). We compared and validated the models with large-scale, high-spatiotempora

SUBMITTER: Obien MEJ 

PROVIDER: S-EPMC6349853 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature