TRPC5 ion channel permeation promotes weight gain in hypercholesterolaemic mice.

ABSTRACT: Transient Receptor Potential Canonical 5 (TRPC5) is a subunit of a Ca²⁺-permeable non-selective cationic channel which negatively regulates adiponectin but not leptin in mice fed chow diet. Adiponectin is a major anti-inflammatory mediator and so we hypothesized an effect of TRPC5 on the inflammatory condition of atherosclerosis. Atherosclerosis was studied in aorta of ApoE^-/- mice fed western-style diet. Inhibition of TRPC5 ion permeation was achieved by conditional transgenic expression of a dominant negative ion pore mutant of TRPC5 (DNT5). Gene expression analysis in adipose tissue suggested that DNT5 increases transcript expression for adiponectin while decreasing transcript expression of the inflammatory mediator Tnfα and potentially decreasing Il6, Il1β and Ccl

SUBMITTER: Rode B 

PROVIDER: S-EPMC6349875 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature