Changes in long-range rDNA-genomic interactions associate with altered RNA polymerase II gene programs during malignant transformation.

ABSTRACT: The three-dimensional organization of the genome contributes to its maintenance and regulation. While chromosomal regions associate with nucleolar ribosomal RNA genes (rDNA), the biological significance of rDNA-genome interactions and whether they are dynamically regulated during disease remain unclear. rDNA chromatin exists in multiple inactive and active states and their transition is regulated by the RNA polymerase I transcription factor UBTF. Here, using a MYC-driven lymphoma model, we demonstrate that during malignant progression the rDNA chromatin converts to the open state, which is required for tumor cell survival. Moreover, this rDNA transition co-occurs with a reorganization of rDNA-genome contacts which correlate with gene expression changes at associated loci, impacting gene on

SUBMITTER: Diesch J 

PROVIDER: S-EPMC6349880 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature