Mfd protects against oxidative stress in Bacillus subtilis independently of its canonical function in DNA repair.

ABSTRACT:

Background

Previous reports showed that mutagenesis in nutrient-limiting conditions is dependent on Mfd in Bacillus subtilis. Mfd initiates one type of transcription-coupled repair (TCR); this type of repair is known to target bulky lesions, like those associated with UV exposure. Interestingly, the roles of Mfd in repair of oxidative-promoted DNA damage and regulation of transcription differ. Here, we used a genetic approach to test whether Mfd protected B. subtilis from exposure to two different oxidants.

Results

Wild-type cells survived tert-butyl hydroperoxide (t-BHP) exposure significantly better than Mfd-deficient cells. This protective effect was independent of UvrA, a component of the canonical TCR/nucleotide excision repair (NER) pathway. Further, our results sugges

SUBMITTER: Martin HA 

PROVIDER: S-EPMC6350366 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature