Biosensor-guided improvements in salicylate production by recombinant Escherichia coli.

ABSTRACT:

Background

Salicylate can be biosynthesized from the common metabolic intermediate shikimate and has found applications in pharmaceuticals and in the bioplastics industry. While much metabolic engineering work focused on the shikimate pathway has led to the biosynthesis of a variety of aromatic compounds, little is known about how the relative expression levels of pathway components influence salicylate biosynthesis. Furthermore, some host strain gene deletions that improve salicylate production may be impossible to predict. Here, a salicylate-responsive transcription factor was used to optimize the expression levels of shikimate/salicylate pathway genes in recombinant E. coli, and to screen a chromosomal transposon insertion library for improved salicylate production.

Results</

SUBMITTER: Qian S 

PROVIDER: S-EPMC6350385 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature