Differential Expression and Significance of PD-L1, IDO-1, and B7-H4 in Human Lung Cancer.

ABSTRACT:

Purpose

To determine the expression level, associations, and biological role of PD-L1, IDO-1, and B7-H4 in non-small cell lung cancer (NSCLC).

Experimental design

Using multiplexed quantitative immunofluorescence (QIF), we measured the levels of PD-L1, IDO-1, B7-H4, and different tumor-infiltrating lymphoycte (TIL) subsets in 552 stages I-IV lung carcinomas from two independent populations. Associations between the marker levels, TILs, and major clinicopathologic variables were determined. Validation of findings was performed using mRNA expression data from The Cancer Genome Atlas (TCGA) and in vitro stimulation of lung adenocarcinoma A549 cells with IFNγ and IL10.

Results

PD-L1 was detected in 16.9% and 21.8% of cases in each population. IDO-1 was expressed in 42.6%

SUBMITTER: Schalper KA 

PROVIDER: S-EPMC6350535 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature