Evaluating natural killer cell cytotoxicity against solid tumors using a microfluidic model.

ABSTRACT: Immunotherapies against solid tumors face additional challenges compared with hematological cancers. In solid tumors, immune cells and antibodies need to extravasate from vasculature, find the tumor, and migrate through a dense mass of cells. These multiple steps pose significant obstacles for solid tumor immunotherapy and their study has remained difficult using classic in vitro models based on Petri dishes. In this work, a microfluidic model has been developed to study natural killer cell response. The model includes a 3D breast cancer spheroid in a 3D extracellular matrix, and two flanking lumens lined with endothelial cells, replicating key structures and components during the immune response. Natural Killer cells and antibodies targeting the tumor cells were either embedded in

SUBMITTER: Ayuso JM 

PROVIDER: S-EPMC6350694 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature