Membrane-potential compensation reveals mitochondrial volume expansion during HSC commitment.

ABSTRACT: Proper control of mitochondrial function is a key factor in the maintenance of hematopoietic stem cells (HSCs). Mitochondrial content is commonly measured by staining with fluorescent cationic dyes. However, dye staining can be affected, not only by xenobiotic efflux pumps, but also by dye intake, which is dependent on the negative charge of mitochondria. Therefore, mitochondrial membrane potential (ΔΨ_mt) must be considered in these measurements because a high ΔΨ_mt due to respiratory chain activity can enhance dye intake, leading to the overestimation of mitochondrial volume. Here, we show that HSCs exhibit the highest ΔΨ_mt of the hematopoietic lineages and, as a result, ΔΨ_mt-independent methods most accurately assess the relatively low mitochond

SUBMITTER: Bonora M 

PROVIDER: S-EPMC6350772 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature