Quantitative phosphoproteomic analysis of the molecular substrates of sleep need.

ABSTRACT: Sleep and wake have global effects on brain physiology, from molecular changes^1-4 and neuronal activities to synaptic plasticity^3-7. Sleep-wake homeostasis is maintained by the generation of a sleep need that accumulates during waking and dissipates during sleep^8-11. Here we investigate the molecular basis of sleep need using quantitative phosphoproteomic analysis of the sleep-deprived and Sleepy mouse models of increased sleep need. Sleep deprivation induces cumulative phosphorylation of the brain proteome, which dissipates during sleep. Sleepy mice, owing to a gain-of-function mutation in the Sik3 gene ¹² , have a constitutively high sleep need despite increased sleep amount. The brain proteome of these mice exhibits hyperphosphorylation, simil

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6350790 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature