Neuropeptides PDF and DH31 hierarchically regulate free-running rhythmicity in Drosophila circadian locomotor activity.

ABSTRACT: Neuropeptides play pivotal roles in modulating circadian rhythms. Pigment-dispersing factor (PDF) is critical to the circadian rhythms in Drosophila locomotor activity. Here, we demonstrate that diuretic hormone 31 (DH31) complements PDF function in regulating free-running rhythmicity using male flies. We determined that Dh31 loss-of-function mutants (Dh31^#51) showed normal rhythmicity, whereas Dh31^#51;Pdf⁰¹ double mutants exhibited a severe arrhythmic phenotype compared to Pdf-null mutants (Pdf⁰¹). The expression of tethered-PDF or tethered-DH31 in clock cells, posterior dorsal neurons 1 (DN1ps), overcomes the severe arrhythmicity of Dh31^#51;Pdf⁰¹ double mutants, suggesting that DH31 and PDF may act on DN1ps to regulate fre

SUBMITTER: Goda T 

PROVIDER: S-EPMC6351594 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature