Rapid diagnosis of Mycoplasma pneumonia infection by denaturation bubble-mediated strand exchange amplification: comparison with LAMP and real-time PCR.

ABSTRACT: M. pneumoniae infection is often ignored due to its similar clinical symptom with respiratory tract infections caused by bacteria or viruses, and thus leading to misdiagnosis and delayed treatment. It is critical to develop a rapid, sensitive and specific diagnosis method. Denaturation Bubble-mediated Strand Exchange Amplification (SEA) was established, which is an isothermal method with only a primer pair and one Bst DNA polymerase. Notably, colorimetric SEA assay was developed with simple visual readout, making instrument-independent in detection step. The method could detect as low as 1.0 × 10⁴ copies/mL genomic DNA within 60 min. Considering that more than 80% infected patients have 1.0 × 10⁵-1.0 × 10⁷ copies/mL M. pneumonia DNA, SEA is available for th

SUBMITTER: Shi W 

PROVIDER: S-EPMC6351683 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature