Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors.

ABSTRACT: The derivation of kidney tissues from human pluripotent stem cells (hPSCs) and its application for replacement therapy in end-stage renal disease have been widely discussed. Here we report that consecutive transfections of two sets of synthetic mRNAs encoding transcription factors can induce rapid and efficient differentiation of hPSCs into kidney tissues, termed induced nephron-like organoids (iNephLOs). The first set - FIGLA, PITX2, ASCL1 and TFAP2C, differentiated hPSCs into SIX2⁺SALL1⁺ nephron progenitor cells with 92% efficiency within 2 days. Subsequently, the second set - HNF1A, GATA3, GATA1 and EMX2, differentiated these cells into PAX8⁺LHX1⁺ pretubular aggregates in another 2 days. Further culture in both 2-dimensional and 3-dimensional

SUBMITTER: Hiratsuka K 

PROVIDER: S-EPMC6351687 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature