Identification and Verification of Ubiquitin-Activated Bacterial Phospholipases.

ABSTRACT: ExoU is a potent type III secretion system effector that is injected directly into mammalian cells by the opportunistic pathogen Pseudomonas aeruginosa As a ubiquitin-activated phospholipase A₂ (PLA₂), ExoU exhibits cytotoxicity by cleaving membrane phospholipids, resulting in lysis of the host cells and inhibition of the innate immune response. Recently, ExoU has been established as a model protein for a group of ubiquitin-activated PLA₂ enzymes encoded by a variety of bacteria. Bioinformatic analyses of homologous proteins is a powerful approach that can complement and enhance the overall understanding of protein structure and function. To conduct homology studies, it is important to have efficient and effective tools to screen and to validate the

SUBMITTER: Tessmer MH 

PROVIDER: S-EPMC6351748 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature