Genetic Determinants and Prediction of Antibiotic Resistance Phenotypes in Helicobacter pylori.

ABSTRACT: Helicobacter pylori is a major human pathogen. Diagnosis of H. pylori infection and determination of its antibiotic susceptibility still mainly rely on culture and phenotypic drug susceptibility testing (DST) that is time-consuming and laborious. Whole genome sequencing (WGS) has recently emerged in medical microbiology as a diagnostic tool for reliable drug resistance prediction in bacterial pathogens. The aim of this study was to compare phenotypic DST results with the predictions based on the presence of genetic determinants identified in the H. pylori genome using WGS. Phenotypic resistance to clarithromycin, metronidazole, tetracycline, levofloxacin, and rifampicin was determined in 140 clinical H. pylori isolates by E-Test^®, and the occurrence o

SUBMITTER: Lauener FN 

PROVIDER: S-EPMC6351930 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature