Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway.

ABSTRACT:

Background

The combination of Radix Paeoniae Alba (RPA) and Rhizoma Atractylodis Macrocephalae (RAM) has long been used as a classic herb pair for the treatment of gynecologic and gastrointestinal diseases, but the underlying mechanisms of the herb pair remain unknown. This study aims to explore the anti-inflammatory potentials of RPA-RAM herb pair and to elucidate the underlying mechanisms.

Methods

The bioactive parts of RPA-RAM were extracted and screened through the inhibitory effects against nitric oxide (NO) production. The effects of optimized RPA-RAM extracts (OPAE) on inflammation-associated mediators were investigated by Western blotting, real-time quantitative PCR (RT-qPCR), Enzyme-linked immunosorbent (ELISA) and immunofluorescence staining.

Results

OPAE po

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC6352364 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature