Isolation and identification of a vegetative organ-specific promoter from maize.

ABSTRACT: To avoid the unregulated overexpression of the exogenous genes, specific or inducible expression is necessary for some exogenous genes in transgenic plants. But little is known about organ- or tissue-specific promoters in maize. In the present study, the expression of a maize pentatricopeptide repeat (PPR) protein encoding gene, GRMZM2G129783, was analyzed by RNA-sequencing data and confirmed by quantitative real time PCR. The results showed that the PPR _{GRMZM2G129783} gene specifically expressed in vegetative organs. Consequently, a 1830 bp sequence upstream of the start codon of the promoter for GRMZM2G129783 gene was isolated from maize genome (P ₁₈₃₀ ). To validate whether the promoter possesses the vegetative organ-specifi

SUBMITTER: Yu H 

PROVIDER: S-EPMC6352524 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature