Molecular characterization and expression analysis of iron superoxide dismutase gene from Pseudochlorella pringsheimii (Trebouxiophyceae, Chlorophyta).

ABSTRACT: The FeSOD isoforms of Pseudochlorella pringsheimii were identified, a preliminary characterization of the enzyme was conducted, and the relationship among the FeSOD gene from P. pringsheimii and that of other organisms was examined. The FeSOD has an open reading frame of 612 bp that encodes 203 deduced amino acids with a molecular mass of 23 kDa. Expression of the recombinant FeSOD gene was done successfully in Escherichia coli. The purified FeSOD has a specific enzyme activity that reached 688 U mg^-1 protein (in vitro assay). Alkaline conditions showed the highest activity for the recombinant FeSOD. Moreover, it showed a relative thermostability up to 50 °C, while at 50 and 70 °C, the activity was reduced by 32 and 68%, respectively, after 1 h as compared t

SUBMITTER: Ismaiel MMS 

PROVIDER: S-EPMC6352527 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature