Screening internal controls for expression analyses involving numerous treatments by combining statistical methods with reference gene selection tools.

ABSTRACT: Real-time PCR is always the method of choice for expression analyses involving comparison of a large number of treatments. It is also the favored method for final confirmation of transcript levels followed by high throughput methods such as RNA sequencing and microarray. Our analysis comprised 16 different permutation and combinations of treatments involving four different Agrobacterium strains and three time intervals in the model plant Arabidopsis thaliana. The routinely used reference genes for biotic stress analyses in plants showed variations in expression across some of our treatments. In this report, we describe how we narrowed down to the best reference gene out of 17 candidate genes. Though we initiated our reference gene selection process using common tools such as

SUBMITTER: Joseph JT 

PROVIDER: S-EPMC6352529 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature