Effect of elicitors on the metabolites in the suspension cell culture of Salvia miltiorrhiza Bunge.

ABSTRACT: The effect of elicitors on the metabolites in the suspension cells of Salvia miltiorrhiza Bunge was elucidated by comparing and analyzing the metabolites of induced and uninduced suspension cells. The primary metabolites were detected by GC-MS. Twelve types of secondary metabolites, namely, shikimic acid, tanshinol, protocatechuic acid, caffeic acid, p-coumaric acid, rosmarinic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone, cryptotanshinone, tanshinone I, and tanshinone II A were detected by HPLC. Results indicated a total of 90 primary metabolites in the cells. These metabolites consisted of 49 kinds of sugars and their derivatives, 15 organic acids, 9 amino acids and their derivatives, 9 hydrocarbons, and 8 other substances. OPLS-DA results indicated five

SUBMITTER: Yu Y 

PROVIDER: S-EPMC6352533 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature