Comparative analysis of KPC-2-encoding chimera plasmids with multi-replicon IncR:Inc_pA1763-KPC:IncN1 or IncFII_pHN7A8:Inc_pA1763-KPC:IncN1.

ABSTRACT:

Background

IncR, IncFII, IncpA1763-KPC, and IncN1 plasmids have been increasingly found among Enterobacteriaceae species, but plasmids with hybrid structures derived from the above-mentioned incompatibility groups have not yet been described.

Methods

Plasmids p721005-KPC, p504051-KPC, and pA3295-KPC were fully sequenced and compared with previously sequenced related plasmids pHN84KPC (IncR), pKPHS2 (IncFII_K), pKOX_NDM1 (IncFII_Y), pHN7A8 (IncFII_pHN7A8), and R46 (IncN1).

Results

The backbone of p721005-KPC/p504051-KPC was a hybrid of the entire 10-kb IncR-type backbone from pHN84KPC, the entire 64.3-kb IncFII_K-type maintenance, and conjugal transfer regions from pKPHS2, a 15.5-kb IncFII_Y-type maintenance regi

SUBMITTER: Qu D 

PROVIDER: S-EPMC6353027 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature