Collective radioresistance of T47D breast carcinoma cells is mediated by a Syncytin-1 homologous protein.

ABSTRACT: It is generally accepted that radiotherapy must target clonogenic cells, i.e., those cells in a tumour that have self-renewing potential. Focussing on isolated clonogenic cells, however, may lead to an underestimate or even to an outright neglect of the importance of biological mechanisms that regulate tumour cell sensitivity to radiation. We develop a new statistical and experimental approach to quantify the effects of radiation on cell populations as a whole. In our experiments, we change the proximity relationships of the cells by culturing them in wells with different shapes, and we find that the radiosensitivity of T47D human breast carcinoma cells in tight clusters is different from that of isolated cells. Molecular analyses show that T47D cells express a Syncytin-1 homologous protei

SUBMITTER: Chignola R 

PROVIDER: S-EPMC6353071 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature