Statistical optimization of crude oil bio-degradation by a local marine bacterium isolate Pseudomonas sp. sp48.

ABSTRACT: Pseudomonas sp. sp48, a marine bacterium isolated from Bahary area (Alexandria, Egypt), showed a high potency for oil degradation up to 1.5%. Additionally, it showed an ability to consume aromatic hydrocarbons (phenol & naphthalene) and aliphatic (pentadecane) reaching to 79; 73; 62%, respectively. In the current study, Plackett-Burman factorial design was applied to evaluate culture conditions affecting the degradation potency. Analysis of Plackett-Burman design results revealed that, the most significant variables affecting oil removal were magnesium sulfate, inoculum size, glucose and Triton X-100. To optimize the levels of these significant variables Response Surface Methodology (RSM) was followed. In this respect, the three-level Box-Behnken design was employed and a polynomial model

SUBMITTER: Farag S 

PROVIDER: S-EPMC6353655 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature