Characterizing and alleviating ion suppression effects in atmospheric pressure matrix-assisted laser desorption/ionization.

ABSTRACT:

Rationale

As a powerful ambient ion source, atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) enables direct analysis at atmospheric pressure/temperature and minimal sample preparation. With the increasing usage of AP-MALDI sources with Orbitrap instruments, systematic characterization of the extent of ion suppression effect (ISE) in AP-MALDI-Orbitrap mass spectrometry imaging (MSI) is desirable. Recently, a new low-pressure MALDI platform has been introduced that reportedly provided better sensitivity. While extensive research efforts have been devoted to improving spatial resolution, fewer studies focused on the characterization and sensitivity improvement of these MALDI platforms that, coupled with high-resolution Orbitraps, provide powerful strategy

SUBMITTER: Li G 

PROVIDER: S-EPMC6353668 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature