High- and low-dose oral immunotherapy similarly suppress pro-allergic cytokines and basophil activation in young children.

ABSTRACT:

Background

Mechanisms underlying oral immunotherapy (OIT) are unclear and the effects on immune cells at varying maintenance doses are unknown.

Objective

We aimed to determine the immunologic changes caused by peanut OIT in preschool aged children and determine the effect on these immune responses in groups ingesting low or high-dose peanut OIT (300 mg or 3000 mg, respectively) as maintenance therapy.

Methods

Blood was drawn at several time-points throughout the OIT protocol and PBMCs isolated and cultured with peanut antigens. Secreted cytokines were quantified via multiplex assay, whereas Treg and peanut-responsive CD4 T cells were studied with flow cytometry. Basophil activation assays were also conducted.

Results

Th2-, Th1-, Th9- and Tr1-type cytokines decr

SUBMITTER: Kulis M 

PROVIDER: S-EPMC6353702 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature