Plant regeneration, developmental pattern and genetic fidelity of somatic embryogenesis derived Musa spp.

ABSTRACT: Multiplication of banana cvs. Grand Naine (Musa AAA, Cavendish-sub group) and Rasthali (Musa AAB, Silk-sub group) were attempted through somatic embryogenesis. The influence of position of male flower buds, amino acid supplements in the induction of somatic embryogenesis and field performance of embryogenic cell suspension (ECS) derived banana plants were studied. Differentiated immature male flower buds positioned at 6-8 th bract whorl as explants showed better callus induction and somatic embryogenesis. Supplementation with glutamine at 400 mg L^-1 along with 20:20 g L^-1sucrose: maltose in maturation media induced a 10-fold increase in somatic embryo formation compared to control. Cotyledonary stage somatic embryos desiccated for 2 h showed higher germi

SUBMITTER: Nandhakumar N 

PROVIDER: S-EPMC6353768 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature