TCR Repertoire Analysis Reveals Mobilization of Novel CD8⁺ T Cell Clones Into the Cancer-Immunity Cycle Following Anti-CD4 Antibody Administration.

ABSTRACT: Depletion of CD4⁺ cells using an anti-CD4 monoclonal antibody (anti-CD4 mAb) induces the expansion of tumor-reactive CD8⁺ T cells and strong antitumor effects in several murine tumor models. However, it is not known whether the anti-CD4 mAb treatment activates a particular or a broad spectrum of tumor-reactive CD8⁺ T cell clones. To investigate the changes in the TCR repertoire induced by the anti-CD4 mAb treatment, we performed unbiased high-throughput TCR sequencing in a B16F10 mouse subcutaneous melanoma model. By Inter-Organ Clone Tracking analysis, we demonstrated that anti-CD4 mAb treatment increased the diversity and combined frequency of CD8⁺ T cell clones that overlapped among the tumor, draining lymph node (dLN), and peripheral blood re

SUBMITTER: Aoki H 

PROVIDER: S-EPMC6353793 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature