Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.

ABSTRACT: High-resolution molecular programmes delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation embryos are largely inaccessible. Notwithstanding, we previously suggested conserved principles of pig and human early development. For further insight on pluripotent states and lineage delineation, we analysed pig embryos at single cell resolution. Here we show progressive segregation of inner cell mass and trophectoderm in early blastocysts, and of epiblast and hypoblast in late blastocysts. We show that following an emergent short naive pluripotent signature in early embryos, there is a protracted appearance of a primed signature in advanced embryonic stages. Dosa

SUBMITTER: Ramos-Ibeas P 

PROVIDER: S-EPMC6353908 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature