Trans-β-galactosidase activity of pig enzymes embedded in the small intestinal brush border membrane vesicles.

ABSTRACT: This work highlights the utility of brush border membrane vesicles (BBMV) from the pig small intestine as a reliable model for gathering information about the reaction mechanisms involved in the human digestion of dietary carbohydrates. Concretely, the elucidation of the transgalactosylation mechanism of pig BBMV to synthesize prebiotic galacto-oligosaccharides (GOS) is provided, unravelling the catalytic activity of mammalian small intestinal β-galactosidase towards the hydrolysis of GOS. This study reveals that pig BBMV preferably synthesizes GOS linked by β-(1 → 3) bonds, since major tri- and disaccharide were produced by the transfer of a galactose unit to the C-3 of the non-reducing moiety of lactose and to the C-3 of glucose, respectively. Therefore, these results point out that diet

SUBMITTER: Julio-Gonzalez LC 

PROVIDER: S-EPMC6353940 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature