Template-directed RNA polymerization and enhanced ribozyme catalysis inside membraneless compartments formed by coacervates.

ABSTRACT: Membraneless compartments, such as complex coacervates, have been hypothesized as plausible prebiotic micro-compartments due to their ability to sequester RNA; however, their compatibility with essential RNA World chemistries is unclear. We show that such compartments can enhance key prebiotically-relevant RNA chemistries. We demonstrate that template-directed RNA polymerization is sensitive to polycation identity, with polydiallyldimethylammonium chloride (PDAC) outperforming poly(allylamine), poly(lysine), and poly(arginine) in polycation/RNA coacervates. Differences in RNA diffusion rates between PDAC/RNA and oligoarginine/RNA coacervates imply distinct biophysical environments. Template-directed RNA polymerization is relatively insensitive to Mg²⁺ concentration when performe

SUBMITTER: Poudyal RR 

PROVIDER: S-EPMC6353945 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature