Experimental Applications of in situ Liver Perfusion Machinery for the Study of Liver Disease.

ABSTRACT: The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahepatic IVC. In situ liver perfusion can be utilized t

SUBMITTER: Choi WM 

PROVIDER: S-EPMC6354060 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature