The antimicrobial protein S100A12 identified as a potential autoantigen in a subgroup of atopic dermatitis patients.

ABSTRACT:

Background

Atopic dermatitis (AD) is a complex heterogeneous chronic inflammatory skin disease. Specific IgE antibodies against autoantigens have been observed in a subgroup of AD patients, however, little is known about IgG-auto-reactivity in AD. To investigate the presence of autoreactive IgG antibodies, we performed autoantibody profiling of IgG in patients with AD of different severities and in healthy controls (HC).

Methods

First, we performed an untargeted screening in plasma samples from 40 severe AD (sAD) patients and 40 HC towards 1152 protein fragments on planar antigen microarrays. Next, based on the findings and addition of more fragments, a targeted antigen suspension bead array was designed to profile a cohort of 50 sAD patients, 123 patients with moderate AD (

SUBMITTER: Mikus M 

PROVIDER: S-EPMC6354350 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature