Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis.

ABSTRACT:

Background

Lysyl oxidase-like 4 (LOXL4) has been found to be dysregulated in several human malignancies, including hepatocellular carcinoma (HCC). However, the role of LOXL4 in HCC progression remains largely unclear. In this study, we investigated the clinical significance and biological involvement of LOXL4 in the progression of HCC.

Methods

LOXL4 expression was measured in HCC tissues and cell lines. Overexpression, shRNA-mediated knockdown, recombinant human LOXL4 (rhLOXL4), and deletion mutants were applied to study the function of LOXL4 in HCC. Exosomes derived from HCC cell lines were assessed for the ability to promote cancer progression in standard assays. The effects of LOXL4 on the FAK/Src pathway were examined by western blotting.

Results

LOXL4 was commonl

SUBMITTER: Li R 

PROVIDER: S-EPMC6354392 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature