Dynamic expression and functional analysis of circRNA in granulosa cells during follicular development in chicken.

ABSTRACT:

Background

Circular RNA (circRNA) is a type of noncoding RNA involved in a variety of biological processes, especially in post-transcriptional regulation. The granulosa cells of follicles play a determining role in ovarian development. However, the function of circRNA in chicken follicles is unclear. To better understand the molecular mechanism underlying follicular development and granulosa cell function, we performed a strategy of second-generation sequencing and linear RNA depletion for granulosa cells from small yellow follicles (SYF, 5-8 mm), the smallest hierarchal follicles (F6, 9-12 mm), and the largest hierarchal follicles (F1, ~ 40 mm).

Results

We predicted a total of 11,642 circRNAs that distributed on almost all chromosomes. The majority of the splice lengths of

SUBMITTER: Shen M 

PROVIDER: S-EPMC6354403 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature