Molecular identification of a root apical cell-specific and stress-responsive enhancer from an Arabidopsis enhancer trap line.

ABSTRACT:

Background

Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressing reporters (GFP) only in the root tip was adopted in this study to isolate a DNA fragment.

Results

Using nested PCR, DNA sequencing and sequence homology search, the T-DNA insertion site(s) and its flanking genes were characterised in J3411 line. Subsequently, a 2000 bp plant DNA-fragment (E_rtip1) upstream of the insert position of the coding T-DNA was in silico analysed, revealing certain putative promoter/enhancer cis-regulatory ele

SUBMITTER: Zhang L 

PROVIDER: S-EPMC6354418 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature