Medium- to long-term survival and functional examination of human iPSC-derived retinas in rat and primate models of retinal degeneration.

ABSTRACT:

Background

We have previously reported that xeno-transplanted human ESC-derived retinas are able to mature in the immunodeficient retinal degeneration rodent models, similar to allo-transplantations using mouse iPSC-derived retina. The photoreceptors in the latter developed outer segments and formed synapses with host bipolar cells, driving light responses of host retinal ganglion cells. In view of clinical application, here we further confirmed the competency of human iPSC-derived retina (hiPSC-retina) to mature in the degenerated retinas of rat and monkey models.

Methods

Human iPSC-retinas were transplanted in rhodopsin mutant SD-Foxn1 Tg(S334ter)3LavRrrc nude rats and two monkeys with laser-induced photoreceptor degeneration. Graft maturation was studied by immunohistoche

SUBMITTER: Tu HY 

PROVIDER: S-EPMC6354559 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature