Serum and Serum Albumin Inhibit in vitro Formation of Neutrophil Extracellular Traps (NETs).

ABSTRACT: The formation of neutrophil extracellular traps (NETs) is an immune defense mechanism of neutrophilic granulocytes. Moreover, it is also involved in the pathogenesis of autoimmune, inflammatory, and neoplastic diseases. For that reason, the process of NET formation (NETosis) is subject of intense ongoing research. In vitro approaches to quantify NET formation are commonly used and involve neutrophil stimulation with various activators such as phorbol 12-myristate 13-acetate (PMA), lipopolysaccharides (LPS), or calcium ionophores (CaI). However, the experimental conditions of these experiments, particularly the media and media supplements employed by different research groups, vary considerably, rendering comparisons of results difficult. Here, we present the first standardized investigatio

SUBMITTER: Neubert E 

PROVIDER: S-EPMC6354573 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature