Spatial regulation of gene expression during growth of articular cartilage in juvenile mice.

ABSTRACT:

Background

In juvenile mammals, the epiphyses of long bones grow by chondrogenesis within the articular cartilage. A better understanding of the molecular mechanisms that regulate the growth of articular cartilage may give insight into the antecedents of joint disease, such as osteoarthritis.

Methods

We used laser capture microdissection to isolate chondrocytes from the superficial, middle, and deep zones of growing tibial articular cartilage in the 1-wk-old mouse and then investigated expression patterns by microarray. To identify molecular markers for each zone of the growing articular cartilage, we found genes showing zone-specific expression and confirmed by real-time PCR and in situ hybridization.

Results

Bioinformatic analyses implicated ephrin receptor signalin

SUBMITTER: Lui JC 

PROVIDER: S-EPMC6354579 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature