Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers.

ABSTRACT: Cyclospora cayetanensis is a human parasite transmitted via ingestion of contaminated food or water. Cases of C. cayetanensis infection acquired in the United States often go unexplained, partly because of the difficulties associated with epidemiologic investigations of such cases and the lack of genotyping methods. A Multilocus Sequence Typing (MLST) method for C. cayetanensis based on five microsatellite loci amplified by nested PCR was described in 2016. The MLST loci had high variability, but many specimens could not be assigned a type because of poor DNA sequencing quality at one or more loci. We analyzed Cyclospora-positive stool specimens collected during 1997-2016 from 54 patients, including 51 from the United States. We noted limited inter-specimen variability for one locus (CYC15

SUBMITTER: Hofstetter JN 

PROVIDER: S-EPMC6354607 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature