Detection of Concordance between Transcriptional Levels of GPCRs and Receptor-Activity-Modifying Proteins.

ABSTRACT: A recent phylogenetic analysis showed global co-evolution of G protein-coupled receptors (GPCRs) and receptor-activity-modifying proteins (RAMPs) suggesting global interactions between these two protein families. Experimental validation of these findings is challenging because in humans whereas there are only three genes encoding RAMPs, there are about 800 genes encoding GPCRs. Here, we report an experimental approach to evaluate GPCR-RAMP interactions. As a proof-of-concept experiment, we over-expressed RAMP2 in HEK293T cells and evaluated the effect on the transcriptional levels of 14 representative GPCRs that were selected based on the earlier phylogenetic analysis. We utilized a multiplexed error-correcting fluorescence in situ hybridization (MERFISH) method to detect message levels fo

SUBMITTER: Barbash S 

PROVIDER: S-EPMC6354700 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature