Site-selective protein conjugation at histidine.

ABSTRACT: Site-selective conjugation generally requires both (i) molecular engineering of the protein of interest to introduce a conjugation site at a defined location and (ii) a site-specific conjugation technology. Three N-terminal interferon α2-a (IFN) variants with truncated histidine tags were prepared and conjugation was examined using a bis-alkylation reagent, PEG_(10kDa)-mono-sulfone 3. A histidine tag comprised of two histidines separated by a glycine (His₂-tag) underwent PEGylation. Two more IFN variants were then prepared with the His₂-tag engineered at different locations in IFN. Another IFN variant was prepared with the His-tag introduced in an α-helix, and required three contiguous histidines to ensure that two histidine residues in the correct co

SUBMITTER: Peciak K 

PROVIDER: S-EPMC6354831 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature