MiRNA-101-5p inhibits the growth and aggressiveness of NSCLC cells through targeting CXCL6.

ABSTRACT:

Background

The purpose of this study is to explore the potential biological roles of miR-101-5p in the progression of non-small-cell lung carcinoma (NSCLC).

Methods

The levels of miR-101-5p and chemokine (C-X-C motif) ligand 6 (CXCL6) in NSCLC tissues and cells were detected using the quantitative real-time PCR (qRT-PCR) assay. Proliferation, colony formation, migration and invasion assays were conducted using miR-101-5p-transfected NSCLC cells in vitro. The expression of CXCL6 was measured using immunofluorescence assay. Xenograft model and lung metastasis model were constructed to further reveal the precise roles of miR-101-5p in the lung metastasis and growth of NSCLC cells in vivo.

Results

miR-101-5p was underregulated in NSCLC tissues when compared with that in t

SUBMITTER: Chen Q 

PROVIDER: S-EPMC6355169 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature