Neutralization of HIV subtypes A and D by breast milk IgG from women with HIV infection in Uganda.

ABSTRACT:

Objectives

Among HIV-exposed infants in resource-limited countries, 8-12% are infected postnatally by breastfeeding. However, most of those uninfected at birth remain uninfected over time despite daily exposure to HIV in breast milk. Thus, we assessed the HIV-inhibitory activity of breast milk.

Methods

We measured cross-clade neutralization in activated PBMC of Ugandan subtype A (92UG031) and D (92UG005) primary HIV by breast milk or purified milk IgG and IgA from 25 HIV-infected Ugandan women. Isotype-specific antigen recognition was resolved by immunoblot. We determined HIV subtype from envelope population sequences in cells from 13 milk samples by PCR.

Results

Milk inhibited p24 production by ≥50% (dose-dependent) by subtype A (21/25; 84%) and subtype D (11/25; 44%

SUBMITTER: Palaia JM 

PROVIDER: S-EPMC6355459 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature